基于二维MOFs构建MicroRNA荧光传感器的综合分析化学实验及“赛学结合、三位一体评价”的教学模式设计

doi:10.3866/PKU.DXHX201810003

Abstract

Abstract:

This comprehensive analytical experiment has successfully introduced the frontier of science such as two-dimensional metal organic frameworks (2D MOFs) fabrication and MicroRNA (miRNA) analysis into laboratory teaching for undergraduate and graduate students whose major are chemistry or materials science. It mainly includes the following issues:synthesis and characterization of 2D MOFs, construction of 2D MOFs-DNA fluorescent probe, and developing the miRNA fluorescence biosensor. The experiment takes 10 hours. In the experiment, we adopted a new teaching mode of "game-study combination and trinity evaluation". The students learned a breadth of experimental skills, improved their experimental skills and broadened their academic vision. Moreover, through the combination of competition and teaching, the students' study enthusiasms were greatly stimulated, the spirit of teamwork was cultivated. This experiment and the teaching mode are also suitable for promotion to other comprehensive chemistry laboratory teaching.

Key words: Comprehensive analytical chemistry experiment, Fluorescence sensor, Two-dimensional metal organic frameworks (2D MOFs), MicroRNA, Game-study combination, Trinity evaluation

Ning GAN,Qian YANG. Comprehensive Analytical Chemistry Experiment on MicroRNA Fluorescence Sensor Based on Two Dimensional MOFs Probes and New Teaching Mode of "Game-Study Combination and Trinity Evaluation"[J].University Chemistry, 2019, 34(6): 38-45.

Figures/Tables 4

References 8

1	干宁; 栾倩. 大学化学, 2017, 32 (5), 11.
2	Lu Z. X. ; Zhang L. M. ; Deng Y. ; Li S. ; He N. Y. Nanoscale 2012, 4 (19), 5840. doi: 10.1039/c2nr31497e
3	Shi J. Y. ; Guo J. B. ; Bai G. X. ; Chan C. Y. ; Liu X. ; Ye W. W. ; Hao J. H. ; Chen S. ; Yang M. Biosens. Bioelectron. 2015, 65, 238. doi: 10.1016/j.bios.2014.10.050
4	Robertson N. M. ; Toscano A. E. ; Lamantia V. E. ; Hizir M. S. ; Rana M. ; Balcioglu M. ; Jia S. ; Yigit M. V. Biosens. Bioelectron. 2017, 89, 551. doi: 10.1016/j.bios.2016.02.058
5	Zhao H. Q. ; Qiu G. H. ; Liang Z. ; Li M. M. ; Sun B. ; Qin L. ; Yang S. P. ; Chen W. H. ; Chen J. X. Anal. Chim. Acta 2016, 922, 55. doi: 10.1016/j.aca.2016.03.054
6	Wei X. F. ; Zheng L. Y. ; Luo F. ; Lin Z. Y. ; Guo L. H. ; Qiu B. ; Chen G. N. J. Mater. Chem. B 2013, 1 (13), 1812. doi: 10.1039/c3tb00501a
7	Wu Y. F. ; Han J. Y. ; Xue P. ; Xu R. ; Kang Y. J. Nanoscale 2014, 7 (5), 1753.
8	Zhao M. T. ; Wang Y. X. ; Ma Q. L. ; Huang Y. ; Zhang X. ; Ping J. F. ; Zhang Z. C. ; Lu Q. P. ; Yu Y. F. ; Xu H. ; Zhao Y. L. ; Zhang H. Adv. Mater. 2016, 27 (45), 7372.

[1]	Xinqi Zhan, Zhaohui Zhou. Chemical Mechanism of Nephrogenic Systemic Fibrosis and Its Inhibition with Double Chelation [J]. University Chemistry, 0, (): 2209029-.
[2]	Zhenming Xu, Mingbo Zheng, Zhenhui Liu, Duo Chen, Qingsheng Liu. Experimental Design of Project-Driven Teaching in Computational Materials Science: First-Principles Calculations of the LiFePO₄ Cathode Material for Lithium-Ion Batteries [J]. University Chemistry, 2023, (): 2307022-.
[3]	Liangyu Gong, Jie Wang, Fengyu Du, Lubin Xu, Chuanli Ma, Shihai Yan, Zhuwei Song, Fuheng Liu, Xiuzhong Wang. The Construction and Practice of “One-Point, Two-Lines and ThreeSides” Innovative Experimental Platform [J]. University Chemistry, 2023, (): 2308023-.
[4]	Yongmei Liu, Lisen Sun, Zhen Huang, Tao Tu. Curriculum-Based Ideological and Political Design for the Experiment of Methanol Oxidation to Formaldehyde Catalyzed by Electrolytic Silver [J]. University Chemistry, 2023, (): 2308020-.
[5]	Xiuyun Wang, Jiashuo Cheng, Yiming Wang, Haoyu Wu, Yan Su, Yuzhuo Gao, Xiaoyu Liu, Mingyu Zhao, Chunyan Wang, Miao Cui, Wenfeng Jiang. Improvement of Sodium Ferric Ethylenediaminetetraacetate (NaFeEDTA) Iron Supplement Preparation Experiment [J]. University Chemistry, 2023, (): 2308067-.
[6]	Shuang Meng, Haixin Long, Zhou Zhou, Meizhu Rong. Inorganic Chemistry Curriculum Design and Implementation of Based on “Stepped-Task Driven + Multi-Dimensional Output” Model: A Case Study on Intermolecular Forces [J]. University Chemistry, 2023, (): 2309008-.
[7]	Pingping Zhu, Wei Shao, Qizhi Yao, Yuan Zheng, Wan Li, Lingling Li, Wanquan Zhang, Zhenggen Zha, Ming Guan, Xi Bai, Houjin Li, Yu Hu, Hong Yuan, Kai Li, Wenyuan Hu, Ailing Wang, Junlong Zhao, Cuiping Zhai, Chuanli Qin, Naiying Fan, Ruixiang Hu, Yongwen Shen, Junsheng Hao, Yue Zhang, Faxiong Zhao, Haibo Zhang, Chengbin Gong, Ru Xia, Haigang Hao, Xingwen Zhang, Xingwen Sun, Zhen Gao, Yong Fan, Dongcai Guo, Tian Cao, Shiwei Luo, Hongchun Li, Wanqun Hu, Quan Lan, Hong Wu. Building a Community for Teaching Development, with Multiple Universities Attending One Class Together: Preliminary Exploration and Effect of Teaching and Research Activities Based on Virtual Teaching and Research Section of Chemistry Experiments Course Group [J]. University Chemistry, 0, (): 2309007-.
[8]	Zheng Wang, Xu Peng, Haojie Li, Zhiyue Dong, Hongying Liu, Chi Zhang. Design and Practice of Blended Teaching of Inorganic Chemistry Experiment Based on BOPPPS [J]. University Chemistry, 2023, 38(9): 69-79.
[9]	Hongyan Lu, Tao Yang, Bo Han, Qing Xu, Xiaofei Zeng, Qiaohong Zhu. Learn to Apply and Teach to Achieve: Introducing a Comprehensive Experiment on Photocatalytic Pollutants Removal by TiO₂/g-C₃N₄ [J]. University Chemistry, 2023, 38(9): 131-140.
[10]	Luyao Chen, Zhifei Zhu, Chenyao Li, Youju Huang, Weiming Xu. Integration of Culture and Education, Promoting Conservation through Learning: Taking the Protection and Inheritance of Intangible Cultural Heritage in the Chemistry and Society Course as an Example [J]. University Chemistry, 2023, 38(9): 6-13.
[11]	Aidang Lu, Ruihu Wang, Yunting Liu, Yanjun Jiang. Comprehensive Chemistry Experiment: Nomenclature, Synthesis, and Characterization of Fascaplysin Alkaloids [J]. University Chemistry, 2023, 38(9): 141-147.
[12]	Zhengyan Zhao, Zhaoyin Wang, Liping Xu, Hongli Li, Dayong Chen. Construction of Micro-Lecture in Analytical Chemistry: Taking Titration Jump as an Example [J]. University Chemistry, 2023, 38(9): 14-18.
[13]	Junbin Li, Chenglin Li, Jiaqi Luo, Hui Chen, Zhihe Qing. Synthesis and Characterization of DNA-Mediated Fluorescent Copper Nanoclusters: Recommending A Research-Oriented Comprehensive Chemical Experiment [J]. University Chemistry, 2023, 38(9): 122-130.
[14]	Qiting He. The Chemical Journey of Coffee Beans [J]. University Chemistry, 2023, 38(9): 80-88.
[15]	Qian Cheng, Shujun Li, Jiguo Zhang, Shixue Ren, Qing Li. Construction and Practice for Innovative Teaching Model of "Principles of Chemical Engineering" Course under the New Form of Internet Plus [J]. University Chemistry, 2023, 38(9): 1-5.

Comprehensive Analytical Chemistry Experiment on MicroRNA Fluorescence Sensor Based on Two Dimensional MOFs Probes and New Teaching Mode of "Game-Study Combination and Trinity Evaluation"

RichHTML

PDF

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 8

Related Articles 15

Metrics

Recommended 0