紫外-可见分光光度计的改造及其在实验教学中的应用

doi:10.3866/PKU.DXHX202002057

Abstract

Abstract:

In this paper, the UV-Visible spectrophotometer (SHIMADZU UV-1750) was simply modified to realize the on-line detection of samples and applied to laboratory teaching. The modification is also applicable to SHIMADZU UV-1780 and other models of spectrophotometer. The modification expands the function and improves the utilization rate of the instrument. In the laboratory teaching, the modified UV-Visible spectrophotometer can become a part of the protein collection system (replacing the traditional protein detector and recorder), thus reducing the acquisition cost of laboratory instruments.

Key words: UV-Visible spectrophotometer, Functional expansion, Laboratory teaching

Yuqing Chen, Shuangwen Chen, Yuanyuan Ji. Modification of UV-Visible Spectrophotometer for Application in Laboratory Teaching[J].University Chemistry, 2021, 36(2): 2002057.

Figures/Tables 5

References 19

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序号	设备名称	品牌型号	数量
1	可见分光光度计	上海光谱SP-722	5
2	紫外分光光度计	岛津UV-1750	2
3	紫外分光光度计	岛津UV-1780	3
4	紫外分光光度计	瓦里安Cary50Bio	1

序号	采集周期/s	采集次数	采集时间/min
设置范围	0-1000	11-12001
1	0.1	12001	20
2	0.2	12001	40
3	0.3	12001	60
4	0.4	12001	80
5	0.5	12001	100
6	0.6	12001	120
7	0.7	12001	140
8	0.8	12001	160
9	0.9	12001	180
10	1	12001	200
11	2	12001	400
12	3	12001	600
13	4	12001	800
14	5	12001	1000
15	6	12001	1200
16	10	12001	2000
17	1000	12001	200000

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Modification of UV-Visible Spectrophotometer for Application in Laboratory Teaching

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