University Chemistry ›› 2023, Vol. 38 ›› Issue (1): 155-160.doi: 10.3866/PKU.DXHX202201014

• Chemistry Laboratory • Previous Articles     Next Articles

Determination of Surface Tension Isotherm of Phospholipid Solution Using Wilhelmy Method

Yuanfei Li, Xunfang Yang, Guiling Wang, Ying Guo, Xu Wang, Xinru Li, Yanxia Zhou, Ying Xie()   

  • Received:2022-01-07 Accepted:2022-01-18 Published:2022-03-07
  • Contact: Ying Xie


The determination of surface tension isotherm of surfactants is an important experiment of college physical chemistry laboratory. With the wide application of surfactants and the development of scientific instruments, it is necessary to update the experimental system of classical surface tension isotherm measurements. Herein, the surface tension isotherm of distearoyl phosphatidylcholine (DSPC) and dipalmitoyl phosphatidylcholine (DPPC) were determined by interfacial tensiometer, and the important parameters such as surface excess and cross-sectional area of molecules were calculated. An extended experiment was also suggested so as to promote the students' comprehensive knowledge and cultivate the students' ability to analyze and solve problems.

Key words: Phospholipid, Surface tension isotherm, Laboratory teaching