比较教学法在仪器分析实验教学中的应用

doi:10.3866/PKU.DXHX202309068

Abstract

Abstract:

Instrumental analysis experiment constitutes an essential component for medical prevention and medical testing specialties. To improve the innovative and practical abilities of the undergraduates, a designed experiment of simultaneous determination of acetylsalicylic acid and caffeine in Aspirin tablets by High Performance Liquid Chromatography (HPLC) and Ultra Performance Convergence Chromatography (UPC²) was applied. Additionally, ideological and political elements were organically integrated into the teaching process. Educational practice has shown that by facilitating comparative learning of different instrument principles and functional characteristics, students' interest in experimentation and exploratory spirit can be invigorated. This approach also strengthens students' problem-solving skills, thereby elevating their scientific literacy.

Key words: Instrumental analysis experiment, Acetylsalicylic acid, Caffeine, Comparative pedagogy

Yifan Xie, Liyun Yao, Ruolin Yang, Yuxing Cai, Yujie Jin, Ning Li. Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching[J].University Chemistry, 2024, 39(3): 266-273.

Figures/Tables 9

References 17

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混合标准液浓度X (咖啡因-乙酰水杨酸) (μg∙mL⁻¹)	咖啡因峰面积(Y₁)	乙酰水杨酸峰面积(Y₂)	回归方程	回归方程计算的样品浓度(μg∙mL⁻¹)	每片药中咖啡因和乙酰水杨酸含量(mg)
11.9–30.2	168.80	56.27
23.8–60.4	345.55	115.88	Y₁ = 14.069X + 9.46	13.5	34.1 (咖啡因)
35.6–90.7	516.16	172.94
47.5–121	681.34	228.62
119–302	1681.01	567.31	Y₂ = 1.8745X + 1.65	79.8	202 (乙酰水杨酸)
样品	199.55	151.18

混合标准液浓度X (咖啡因-乙酰水杨酸) (μg∙mL⁻¹)	咖啡因峰面积 (Y₃)	乙酰水杨酸峰面积 (Y₄)	回归方程	回归方程计算的样品浓度(μg∙mL⁻¹)	每片药中咖啡因和乙酰水杨酸含量(mg)
11.9–30.2	28565	8588
23.8–60.4	57846	18093	Y₃ = 2367.9X + 898.76	14.0	35.4 (咖啡因)
35.6–90.7	85612	26992
47.5–121	112872	35501
119–302	282692	91237	Y₄ =303.65X − 616.54	79.8	202 (乙酰水杨酸)
样品	34150	23616

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Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching

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