Abstract:

Myoglobin is not only easily available, but also an inherent complex between protein and porphyrin ligand. Therefore, non-covalent complex of myoglobin was selected as the research model for teaching experiment to lead the senior students to practice the direct screening strategy of enzyme inhibitors by electrospray multistage mass spectrometry. Through comparing the effects of different sample preparation conditions on the MS determination of the complex, including organic solvent content and pH, students will understand the importance of sample preparation for MS analysis and grasp how to use MS/MS technology to achieve online qualitative analysis of functional small molecule ligands. The experiment here has been used for a few years as one of the teaching experiments in "comprehensive chemical biology experiments" course for undergraduates majoring in chemical biology at our college. With continuous teaching practice and optimization of the experiment, some good teaching effects have been achieved.

Key words: Electrospray multistage mass spectrometry, Non-covalent interaction, Myoglobin