Abstract: Digital PCR (Polymerase Chain Reaction) enables highly sensitive nucleic acid detection, allowing for the analysis of individual molecules. With its unique capability for absolute quantitation and high sensitivity, digital PCR has emerged as a powerful tool in biomolecular research. This article presents digital PCR from a first-person perspective, allowing readers to appreciate the evolution, technical principles, and applications of digital PCR during the pandemic.

Key words: Digital PCR, Single-molecule detection, Low-abundance detection, High sensitivity, Absolute quantitation