碳量子点的制备及其在生物巯基化合物检测中的应用——推荐一个大学化学研究型综合实验

doi:10.3866/PKU.DXHX202105020

Abstract

Abstract:

A comprehensive chemical experiment is introduced in the paper, including the synthesis, characterization of carbon quantum dots and the fluorescence detection of biothiol. This experiment not only makes students understand the hot spots in the frontier of science, but also enables students to master the structures, principles and operational skills of UV-Vis and fluorescence spectrometers, which are helpful for the innovative consciousness and comprehensive application ability training.

Key words: Carbon quantum dot, Biothiol, Fluorescence detection, Comprehensive experiment

Ying Guo, Yonghui Shang, Minyan Zheng. Synthesis of Carbon Quantum Dots and the Application in Biothiol Detection[J].University Chemistry, 2021, 36(8): 2105020.

Figures/Tables 6

References 8

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试剂名称	规格	生产厂家	试剂名称	规格	生产厂家
Cys	≥ 98%	上海阿拉丁试剂有限公司	Na₂HPO₄	分析纯	天津市化学试剂三厂
CuSO₄	分析纯	上海国药化学试剂公司	硫酸奎宁	分析纯	上海阿拉丁试剂有限公司
NaH₂PO₄	分析纯	天津市化学试剂三厂	H₂SO₄	分析纯	上海国药化学试剂公司

仪器名称	型号	生产厂家
pH计	PB-10	赛多利斯科学仪器(北京)有限公司
循环水式多用真空泵	SHB-Ⅱ	郑州长城科工贸有限公司
电子天平	BBA124S 224S	赛多利斯科学仪器(北京)有限公司
电热鼓风干燥箱	DHG-9030	上海一恒科学仪器有限公司
冷冻离心机	Z383K	德国Hermle公司
荧光分光光度计	F-4600	日本Hitachi公司
紫外-可见光度计	Lambda 36	美国珀金埃尔默
漩涡混合器	QL-9011	江苏林琪贝尔仪器有限公司
微孔滤膜	0.22 μm	天津市津腾实验设备有限公司的
透析袋	1000D	索莱宝科技有限公司

样品	I	A	η	Φ
硫酸奎宁	805.815	0.035	1.33	0.54
碳点	160.050	0.038	1.33	0.10

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Synthesis of Carbon Quantum Dots and the Application in Biothiol Detection

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