响应面法在仪器分析实验教学中的应用——以响应面法优化HPLC测定饮料苯甲酸的条件为例

doi:10.3866/PKU.DXHX202011040

Abstract

Abstract:

The instrumental analysis course is one of the professional basic courses that combine theory and experiment. In order to improve students' scientific research and innovation ability, "the application of response surface method to the optimization of HPLC conditions for determination of benzoic acid in beverages" is taken as an example. Based on the current situation of the experimental course of "instrumental analysis", the data processing software used in response surface analysis is studied in experimental teaching and helps students understand and be proficient in the experimental design and software of response surface methodology.

Key words: Response surface, Instrumental analysis, Experimental teaching

Jialiexi·Manafu, Janaer·Tuerxun, Yue Wan, Fudong Ma. Application of Response Surface Method in the Teaching of Instrumental Analysis Experiment: Taking the Optimization of HPLC Conditions for Determination of Benzoic Acid in Beverages as an Example[J].University Chemistry, 2021, 36(8): 2011040.

Figures/Tables 4

References 11

1	程玲; 李俊凯. 长江大学学报, 2010, 7 (2), 102.
2	沈玥; 张永忠. 东北农业大学学报, 2011, 9 (1), 119.
3	张玉芬; 韩娜仁花. 内蒙古民族大学学报, 2012, 18 (2), 143.
4	李丽. 西北民族大学学报, 2010, 31 (77), 84.
5	张润楚; 郑海涛; 兰燕. 试验设计与分析及参数优化, 北京: 中国统计出版社, 2003.
6	卢泽湘; 范立维; 廖益强. 福建农林大学学报, 2010, 13 (5), 106. doi: 10.3969/j.issn.1671-6922.2010.05.024
7	廖鹏. 浙江化工, 2018, 49 (1), 44.
8	薛林科. 云南化工, 2019, 46 (5), 75.
9	曹利雅; 曹文博. 化工技术与开发, 2019, 48 (9), 43.
10	徐向宏; 何明珠. 实验设计与Design-Expert、SPSS应用, 北京: 科学出版社, 2010.
11	马少妹; 袁爱群; 白丽娟. 高校实验室工作研究, 2004, 82 (4), 21.

因素	水平
因素	−1	0	1
A/min	5	10	15
B/%	10	12.5	15
C	6	7	8

试验号	A	B	C	Y/(mg∙kg⁻¹)
1	−1	0	−1	135
2	0	−1	1	138
3	0	0	0	140
4	−1	−1	0	135
5	1	0	1	138
6	1	1	0	130
7	0	0	0	140
8	1	0	−1	136
9	1	−1	0	135
10	0	0	0	140
11	−1	0	1	138
12	0	1	1	137
13	0	−1	−1	136
14	0	0	0	140
15	−1	1	0	137
16	0	1	−1	135
17	0	0	0	140

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	106.69	9	11.85	10.06	0.0030	**
A	4.50	1	4.50	3.82	0.0916
B	3.12	1	3.12	2.65	0.1475
C	10.13	1	10.13	8.59	0.022	*
AB	12.25	1	12.25	10.39	0.0146	*
AC	0.25	1	0.25	0.21	0.6591
BC	1.421 × 10⁻¹⁴	1	1.421 × 10⁻¹⁴	1.206 × 10⁻¹⁴	1.0000
A²	31.84	1	31.84	27.02	0.0013	**
B²	37.89	1	37.89	32.15	0.0008	***
C²	1.05	1	1.05	0.89	0.3761
残差	8.25	7	1.18
失拟项	8.25	3	2.75	1.47	0.2504
纯误差	0.000	4	0.000
R² = 0.9282

[1]	Junyu Peng, Feng Wang, Hongmei Yuan, Xiaoli Sun. Exploration of the “Sheep-Flock Effect” Teaching Model Based on Dual Preview: Taking Instrumental Analysis Experiment Courses in Local Universities as an Example [J]. University Chemistry, 2025, 40(9): 310-317.
[2]	Zijun Huang, Feng Wu, Shaofeng Pi, Saijin Huang, Zhengjun Fang. Knowledge Graph-based Development of AI Curriculum for Inorganic Chemistry Experiments and Exploration of New Teaching Paradigm [J]. University Chemistry, 2025, 40(9): 228-237.
[3]	Weizhi Wang, Jieling Qin, Jie Cao. The Necessity of Full-English Course in Instrumental Analysis and the Integration of Ideological and Political Education [J]. University Chemistry, 2025, 40(8): 117-123.
[4]	Yida Zhang, Haixia Zhang. Exploration and Practice of English Teaching in College Chemistry Courses: Taking the Instrumental Analysis Course as an Example [J]. University Chemistry, 2025, 40(7): 79-82.
[5]	Haifeng Ma, Xiaocong Tian, Fengbin Wang, Zhonghua Xi, QingWang. Design of College Chemistry Experiment Based on Product Quality Control: Taking “Optimization of Ferrous Fumarate Synthesis Process” as an Example [J]. University Chemistry, 2025, 40(7): 321-327.
[6]	Qiying Xia, Aixiang Wang, Dengxue Ma, Guokui Liu, Yunzhi Li, Xia Leng, Yaoyao Wei, Guangli Zhou. Science and Technology Empowerment, Production and Education Integration, High-Quality Applied Chemistry Talents Training 12345 Experimental Teaching System Innovation and Practice [J]. University Chemistry, 2025, 40(7): 18-25.
[7]	Fang Li, Xiang Wu, Bing Li, Yougui Li. Design and Practice of Course Ideological and Political Education in Modern Instrumental Analysis Based on the OBE Concept [J]. University Chemistry, 2025, 40(7): 26-33.
[8]	Yan Li, Fei Ding, Jielun Yan, Qingyang Zhou, Zhe Wang, Yifan Shi, Jing Wang, Anna Tang. Improving Instrumental Analytical Chemistry Laboratory Teaching: Developing a Bilingual Classroom to Cultivate Innovative Talents [J]. University Chemistry, 2025, 40(7): 83-89.
[9]	Zufeng Qiu, Jie Ouyang, Yiru Wang, Hengting Yang, Xin Liao, Chi Zhang, Xuanyao Jiang, Shunliu Deng, Zhiwei Lin. Comprehensive Instrument Analysis of “Blind Box” Samples: Analysis of Unknown Substances [J]. University Chemistry, 2025, 40(6): 296-302.
[10]	Shuyong Zhang, Zhong Wei, Gang Ni, Chunyan Sun, Jianping Li, Hualong Xu, Xijiang Han, Qiue Cao, Yanguang Wang, Yi Yang, Yuqiang Ding, Wenqing Zhang. Suggestions on Theoretical and Experimental Teaching Contents of Applied Chemistry Majors in Universities [J]. University Chemistry, 2025, 40(5): 1-8.
[11]	Guangtao Zhao, Zonghuai Liu. Application and Exploration of Old University Scientific Instruments in the Cultivation of Undergraduates’ Innovative Ability [J]. University Chemistry, 2025, 40(5): 383-390.
[12]	Shuhui Li, Rongxiuyuan Huang, Yingming Pan. Electrochemical Synthesis of 2,5-Diphenyl-1,3,4-Oxadiazole: A Recommended Comprehensive Organic Chemistry Experiment [J]. University Chemistry, 2025, 40(5): 357-365.
[13]	Feng Lu, Tao Wang, Qi Wang. Preparation and Characterization of Water-Soluble Silver Nanoclusters: A New Design and Teaching Practice in Materials Chemistry Experiment [J]. University Chemistry, 2025, 40(4): 375-381.
[14]	Pingping Zhang, Dong Xiao, Shiyu Zhou, Chuanqiu Tang. Research and Innovative Practice on the Reform of Instrument Analysis Experimental Teaching System for Talent Cultivation in Modern Industry [J]. University Chemistry, 2025, 40(4): 232-238.
[15]	Zhaoyang Li, Haiyan Zhao, Yali Zhang, Yuan Zhang, Shiqiang Cui. Integration of Nobel Prize Achievements in Analytical Technology with College Instrumental Analysis Course [J]. University Chemistry, 2025, 40(3): 269-276.

Application of Response Surface Method in the Teaching of Instrumental Analysis Experiment: Taking the Optimization of HPLC Conditions for Determination of Benzoic Acid in Beverages as an Example

RichHTML

PDF

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 11

Related Articles 15

Metrics

Recommended 0