Abstract: Chemiluminescence plays a crucial role in biomedical analysis and environmental monitoring. In this study, an experiment is designed to synthesize a derivative of Lofenitine, verify its chemiluminescent properties, and explore its application in the detection of heavy metal ions. This experiment provides a comprehensive training opportunity for students in organic synthesis and separation techniques, as well as the kinetic and thermodynamic principles underlying chemiluminescence. Additionally, it enhances their proficiency in operating fluorescence spectrometers and encourages them to engage in exploratory experiments, such as heavy metal ion detection. The experiment encompasses fundamental concepts from organic, analytical, and environmental chemistry, making it both scientifically rigorous and engaging, thus significantly contributing to the cultivation of students’ innovative spirit.

Key words: Lophine, Chemiluminescence, Metal ion determination, Environmental monitoring