大学化学 >> 2023, Vol. 38 >> Issue (5): 188-192.doi: 10.3866/PKU.DXHX202207021

化学实验 上一篇    下一篇

“葡萄糖的两种电分析化学测定法之比较”仪器分析综合实验设计

郭传恩2, 王敬政1, 王晓蕾1,*()   

  1. 1 山东师范大学化学化工与材料科学学院, 济南 250014
    2 山东政法学院司法鉴定中心, 济南 250014
  • 收稿日期:2022-07-04 录用日期:2022-08-25 发布日期:2022-09-19
  • 通讯作者: 王晓蕾 E-mail:wangxl@sdnu.edu.cn

A Comprehensive Design Experiment of Instrumental Analysis: Comparison of Two Electrochemical Sensors for Determining Glucose

Chuanen Guo2, Jingzheng Wang1, Xiaolei Wang1,*()   

  1. 1 College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China
    2 Judicial Expertise Center, Shandong University of Political Science and Law, Jinan 250014, China
  • Received:2022-07-04 Accepted:2022-08-25 Published:2022-09-19
  • Contact: Xiaolei Wang E-mail:wangxl@sdnu.edu.cn

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摘要：

综合设计性实验是培养大学生独立解决问题能力和创新能力的重要途径。开设“葡萄糖的两种电分析化学测定法之比较”综合设计性实验，大学生通过自主设计化学修饰电极和生物传感器的两种电分析化学传感器来测定葡萄糖，对比两种方法的线性范围、灵敏度和选择性等指标，自主选择合适的方法实现对果汁中葡萄糖含量的测定。本项目能使学生较深入了解仪器分析方法的前沿工作，提升学生学习的兴趣和实验教学的效果，培养学生创新性学习的科研素质。

关键词: 葡萄糖, 仪器分析, 综合设计性实验, 生物传感器, 化学修饰电极

Abstract:

Comprehensive design experiment is an important way to cultivate the abilities of college students to independently solve problems and be innovative. A comprehensive design experiment titled "Comparison of two electrochemical sensor methods for determining glucose" was conducted. Students were asked to independently design two types of electrochemical sensors (i.e., a chemically modified electrode and biosensor) for determining glucose by conducting literature investigations. Comparing the linear ranges, sensitivities, and selectivities of the two sensor methods allowed students to independently choose the appropriate method for determining the glucose content in fruit juice. This project helped students to understand the advanced work involved in instrument-based analysis, improved their interest in learning and effective experimental teaching, and cultivated their scientific research skills through innovative learning.

Key words: Glucose, Instrumental analysis, Comprehensive design experiment, Biosensor, Chemically modified electrode