Abstract: High performance liquid chromatography (HPLC) is one of the important instrument analysis methods. However, in university-level instrumental analysis course, the stationary phase is often explained only briefly, which prevents students from effectively linking the theoretical concepts of the van Deemter equation with the practical structure of chromatographic stationary phases. This article introduces a new type of stationary phase—core-shell stationary phase—and analyzes its application from the development history, separation principle, and structural advantages. Not only does it facilitate students to understand the application of the van Deemter equation, but it can also stimulate their learning interest and exploratory spirit.

Key words: Core-shell stationary phase, Instrumental analysis, High performance liquid chromatography, van Deemter equation, Pedagogical applications