流式细胞仪：原理、应用与光谱技术的革新

doi:10.12461/PKU.DXHX202506070

Abstract

Abstract: Flow cytometry enables the detection of scattered light and fluorescence emitted by cells when illuminated vertically by a laser beam, thereby facilitating comprehensive cellular analysis. The advancements in photoelectric technology have driven it from single-parameter to multi-parameter analysis, culminating in the development of full-spectrum flow cytometry. This evolution allows for the acquisition of extensive biological information from complex samples and has a wide range of influences. The article elaborates on the historical development, principles, and diverse applications of flow cytometry, and emphasizes the innovations in spectral technology. Additionally, it outlines potential future directions for the field.

Key words: Flow cytometry, Principle, Application, Spectral technology innovation, Full-spectrum flow cytometry

Jianna Peng, Hongjie Song, Lichun Zhang, Rui Liu, Yi Lü. Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques[J].University Chemistry, 2026, 41(4): 231-238.

References

[1] S.M. Manohar, P. Shah, A. Nair, Bioanalysis 2021, 13 (3), 185.
[2] J.P. Robinson, Biotechniques 2022, 72 (4), 159.
[3] J.P. Nolan, Cytom. Part A 2022, 101 (10), 812.
[4] S. Sharma, J. Boyer, L. Teyton, Nat. Methods 2024, 21 (5), 740.
[5] J.P. Robinson, Cytom. Part A 2019, 95 (8), 823.
[6] 史亮. 中国设备工程, 2021, No. 12, 13.
[7] 谭国桢. 单激光多色流式细胞仪关键技术研究[博士学位论文]. 长春: 长春理工大学, 2023.
[8] 韩旭东, 张皓楠, 孟燕妮, 刘彦强. 实验室科学, 2010, 13 (3), 158.
[9] A. Adan, G. Alizada, Y. Kiraz, Y. Baran, A. Nalbant, Crit. Rev. Biotechnol. 2017, 37 (2), 163.
[10] 李超, 韩金路, 王玉刚, 孙英凯. 中国实用医药, 2009, 4 (20), 235.
[11] 赵书涛, 武晓东, 王策, 陈永勤, 唐玉国. 现代生物医学进展, 2011, 11 (22), 4378.
[12] 李华, 常莹. 中国医疗器械信息, 2011, 17 (5), 37.
[13] 郑海萍, 李艳辉. 邯郸医学高等专科学校学报, 2003, No. 3, 286.
[14] C.K. Snow, Cytom. Part A 2004, 57 (2), 63.
[15] 魏熙胤, 牛瑞芳. 现代仪器, 2006, No. 4, 8.
[16] 沈二霞. 现代医学仪器与应用, 2008, No. 1, 49.
[17] P.K. Chattopadhyay, A.F. Winters, W.E. Lomas, A.S. Laino, D.M. Woods, Annu. Rev. Anal. Chem. 2019, 12 (1), 411.
[18] W.G. Telford, T. Hawley, F. Subach, V. Verkhusha, R.G. Hawley, Methods 2012, 57 (3), 318.
[19] D. Wlodkowic, W. Telford, J. Skommer, Z. Darzynkiewicz, Methods Cell Biol. 2011, 103, 55.
[20] V. Kapoor, F.V. Subach, V.G. Kozlov, A. Grudinin, V.V. Verkhusha, W.G. Telford, Nat. Methods 2007, 4 (9), 678.
[21] F. Reggeti, D. Bienzle, Vet. Pathol. 2011, 48 (1), 223.
[22] H.T. Maecker, J. Trotter, Cytom. Part A 2006, 69 (9), 1037.
[23] 吴丽娟. 流式细胞术临床应用. 北京: 人民卫生出版社, 2020: 70-84.
[24] J.P. Nolan, D. Condello, E. Duggan, M. Naivar, D. Novo, Cytom. Part A 2013, 83 (3), 253.
[25] Y. Han, Y. Gu, A.C. Zhang, Y. Lo, Lab Chip 2016, 16 (24), 4639.
[26] M. Roederer, Cytometry 2001, 45 (3), 194.
[27] 方晓玲. 光谱流式细胞仪荧光采集系统研制[博士学位论文]. 厦门: 厦门大学, 2018.
[28] L. Li, S. Wang, J. Xue, Y. Lin, L. Su, C. Xue, C. Mao, N. Cai, Y. Tian, S. Zhu, et al. Anal. Chem. 2023, 95 (6), 3423.
[29] M.Y. Chang, J.E. Brune, M. Black, W.A. Altemeier, C.W. Frevert, Am. J. Physiol.-Lung Cell. Mol. Physiol. 2023, 325 (4), L518.
[30] M. Yamamoto, J. P. Robinson, Cytom. Part A 2021, 99 (2), 145.
[31] L. Michelutti, M. Bulfoni, E. Nencioni, J. Microbiol. Methods 2020, 170, 105834.
[32] A. Álvarez-Barrientos, J. Arroyo, R. Cantón, C. Nombela, M. Sánchez-Pérez, Clin. Microbiol. Rev. 2000, 13 (2), 167.
[33] 刘波. 中国医疗设备, 2013, 28 (7), 66.
[34] 陈阳, 徐榕, 司书毅, 何琪杨. 齐鲁药事, 2011, 30 (1), 1.
[35] 黄嘉源, 张子桦, 吴耀冰, 王欣怡, 吴嘉怡, 李继霞, 刘连, 廖钊宏. 齐齐哈尔医学院学报, 2025, 46 (2), 159.
[36] C. Zhang, F.B. Wu, Y.Y. Zhang, X. Wang, X.R. Zhang, J. Anal. At. Spectrom. 2001, 16 (12), 1393.
[37] R. Liu, S.X. Zhang, C. Wei, Z. Xing, S.C. Zhang, X.R. Zhang, Accounts Chem. Res. 2016, 49 (5), 775.
[38] S.C. Bendall, E.F. Simonds, P. Qiu, E.D. Amir, P.O. Krutzik, R. Finck, R.V. Bruggner, R. Melamed, A. Trejo, O.I. Ornatsky, et al. Science 2011, 332 (6030), 687.
[39] N. Nitta, T. Sugimura, A. Isozaki, H. Mikami, K. Hiraki, S. Sakuma, T. Iino, F. Arai, T. Endo, Y. Fujiwaki, et al. Cell 2018, 175 (1), 266.

Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Recommended 0

[1]	Ben Yang, Shukun Shen, Pujun Jin, Yujia Luo, Jianyong Hu. Covalent organic frameworks: emerging organic porous materials [J]. University Chemistry, 2026, 41(4): 264-274.
[2]	Fumei Yang, Yuxin Bai, Zhen Zhang, Jia Luo, Yongchen Gou, Mingli Peng, Guang Jia, Haiming Fan. The Rising Path of MPI: A New Star in Medical Imaging Innovation [J]. University Chemistry, 2026, 41(4): 289-296.
[3]	Shuyong Zhang, Yanzhao Yang, Dongcai Guo, Aidong Zhou, Shaofei Wang, Zhen Huang, Huijuan Ying, Yalan Zhong, Fang Zhao, Yanlong Chang, Xiaohua Wang. Suggestions on Teaching Contents and Requirements of Principle of Chemical Engineering Laboratory for Chemistry Majors of Higher Chemistry Education [J]. University Chemistry, 2026, 41(3): 31-39.
[4]	Huilin Li, Qi Ma, Jin Li, Zihan Yu, Xingyu Liu, Ziyan Zhu, Yuhuan Fu, Junlong Zhao. Red Umbrella as a Bridal Gift: Unveiling the Chemical Secrets of Oil-Paper Umbrella Craftsmanship [J]. University Chemistry, 2026, 41(2): 301-306.
[5]	Zhen Zhang, Jie Liu, Hongwei Kang, Cuibing Bai, Jitang Chen. Exploration of 3D Printing-Assisted Teaching of Chemical Engineering Principles Driven by New Quality Productive Forces [J]. University Chemistry, 2026, 41(2): 21-27.
[6]	Xiangxi Kong, Mingyang He, Yineng Ling, Hui Wang, Qixian Xie, Baili Li, Wurong Ren. Digitally Enhanced Measurement of Ignition Delay Time Measurement for Hypergolic Propellants [J]. University Chemistry, 2026, 41(1): 95-106.
[7]	Wenna Wu, Tao Zhang, Tao He, Kai Feng, Yanyang Han, Shanshan Liu, Huajie Liu, Qingzhong Li, Xin Yang. Research and Application of AI Teaching Assistants in the Blended Teaching of Principles of General Chemistry: A Case Study of “Atomic Structure” [J]. University Chemistry, 2025, 40(9): 245-252.
[8]	Daxin Liang, Yudong Li, Haiyue Yang, Bailing Chen, Zhiming Liu, Chengyu Wang. Reform and Practice of an AI-Empowered “Learning-Understanding-Application” Training Model in Materials Chemistry Education [J]. University Chemistry, 2025, 40(9): 253-263.
[9]	Cheng-an Tao, Jian Huang, Yujiao Li. Exploring the Application of Artificial Intelligence in University Chemistry Laboratory Instruction [J]. University Chemistry, 2025, 40(9): 5-10.
[10]	Kexin Feng, Jie Zhang, Yujia Sun, Qiong Ai, Longchun Li. Synthesis, Purification and Characterization of Acetylferrocene and Ferrocenoylacetone [J]. University Chemistry, 2025, 40(8): 307-314.
[11]	Xianglan Zhang, Jingwen Ma, Junya Cao, Weibin Cai, Zhibing Chang, Jinchang Liu. Curriculum Reform and Practice of Principles of Chemical Engineering Guided by the Concept of “Cultivating Values, Strengthening Foundations and Enhancing Engineering Competence” [J]. University Chemistry, 2025, 40(8): 11-17.
[12]	Liangyu Gong, Yongsheng Niu, Xiuzhong Wang, Lihua Lu, Lubin Xu, Shuaishuai Li, Jie Wang. The Exploration and Practice of Integrating “Agriculture, Science, and Education” to Cultivate Chemistry Talent with a Rich “Agrochemical” Background [J]. University Chemistry, 2025, 40(8): 330-337.
[13]	Tingting Wang, Chufeng Sun, Zhenhua Li, Hongling Wang, Wenfang Wang, Xiaoping Su, Lujuan Cui, Chenjun Wang. Four-Stage Progressive Teaching Innovation in “Chemical Engineering Principles” for Cultivating Practical Engineering Skills [J]. University Chemistry, 2025, 40(7): 112-118.
[14]	Ying Liu, Jia Ji, Yinling Hou, Lilan Guo, Xuan Lv. Selenium’s Journey [J]. University Chemistry, 2025, 40(7): 218-224.
[15]	Nan Wu, Hang Zhang, Lingling Wei, Quan Gu, Haoquan Zheng, Weiqiang Zhang, Rui Cao. Cyanide: Poison or Treasure? [J]. University Chemistry, 2025, 40(7): 177-188.