气相色谱法分离正己烷和环己烷的实验设计与教学探索

doi:10.3866/PKU.DXHX201901021

Abstract

Abstract:

The experiment of separating low toxic n-hexane and cyclohexane by traditional gas chromatography was improved with orthogonal experimental design. The effect of sample quantity, vaporization chamber temperature, detector temperature, column temperature, line velocity and separability of the split ratio were examined. Through the experiment, students could understand the use and maintenance of gas chromatography instruments. At the same time, they could understand separability and column efficiency of chromatography.

Key words: Gas chromatography, Orthogonal experimental design, n-Hexane, Cyclohexane

Tingting LI,Yingai PIAO,Jing ZHANG,Jingdong FENG,Yantao SUN. Experimental Design and Exploration of Separating n-Hexane and Cyclohexane by GC[J].University Chemistry, 2019, 34(7): 73-78.

Figures/Tables 5

References 9

1	肖泽文; 王燕杰; 胡小刚. 大学化学, 2012, 27 (3), 84.
2	Liu C. T. ; Zhang M. ; Yan P. ; Liu H. C. ; Liu X. Y. ; Zhan R. T. ; Abdel-Rehim M. J. Anal. Methods Chem. 2016, 10, 1.
3	王翊如. 大学化学, 2014, 29 (1), 29.
4	张运楚; 姜爱民; 徐红东; 张君捧; 杨红娟. 实验室研究与探索, 2016, 35 (2), 224. doi: 10.3969/j.issn.1006-7167.2016.02.053
5	唐雁雁.含正丁醚的三元体系和四元体系的液液相平衡研究[D].广州:暨南大学, 2013.
6	刘瑞江; 张业旺; 闻崇炜; 汤建. 实验技术与管理, 2010, (9), 52. doi: 10.3969/j.issn.1002-4956.2010.09.016
7	李四生; 李昌安; 宋晓琼; 葛成相; 戴震. 中国卫生检验杂志, 2014, 24 (6), 903.
8	李玉贤; 张佳乐; 刘金浩. 实验室科学, 2011, (2), 105. doi: 10.3969/j.issn.1672-4305.2011.02.035
9	傅玉; 曹霞; 王勇. 疾病监测与控制, 2014, (2), 85.

水平	汽化室温度/℃	柱温/℃	检测器温度/℃	分流比	线速度/(mL∙min⁻¹)	进样量/μL
水平	A	B	C	D	E	F
1	87	45	87	100 : 1	18.5	0.2
2	90	50	90	120 : 1	19.0	0.3
3	93	55	93	150 : 1	19.5	0.4

所在列因素	A	B	C	D	E	F	分离度	峰的个数
实验1	1	1	1	1	1	1	2.229	4
							2.382
							2.2954
实验2	1	2	2	2	2	2	3.405	3
							2.713
实验3	1	3	3	3	3	3	2.735	2
实验4	2	1	1	2	2	3	2.097	4
							2.291
							2.633
实验5	2	2	2	3	3	1	3.416	2
实验6	2	3	3	1	1	2	3.011	2
实验7	3	1	2	1	3	2	2.226	4
							2.404
							2.850
实验8	3	2	3	2	1	3	3.326	3
							2.592
实验9	3	3	1	3	2	1	3.171	2
实验10	1	1	3	3	2	2	2.153	4
							2.362
							2.798
实验11	1	2	1	1	3	3	3.181	2
实验12	1	3	2	2	1	1	3.106	3
实验13	2	1	2	3	1	3	2.109	4
							2.302
							2.745
实验14	2	2	3	1	2	1	3.351	2
实验15	2	3	1	2	3	2	2.726	2
实验16	3	1	3	2	3	1	2.202	4
							2.355
							2.724
实验17	3	2	1	3	1	2	3.457	2
							2.716
实验18	3	3	2	1	2	3	2.889	2
均值1	2.840	2.434	2.837	2.907	2.844	2.998
均值2	2.872	3.175	2.891	2.769	2.875	2.802
均值3	2.838	2.940	2.820	2.872	2.830	2.748
极差	0.035	0.741	0.071	0.138	0.045	0.250

组分	优化前				优化后
组分	t/min	W/min	N	H	t/min	W/min	N	H
正己烷	2.910	0.050	18896	1.588	2.833	0.048	19194	1.563
环己烷	3.205	0.034	21008	1.428	3.119	0.050	21101	1.422

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Experimental Design and Exploration of Separating n-Hexane and Cyclohexane by GC

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