基于指示剂置换法的肝素含量测定——推荐一个分析化学综合设计性实验

doi:10.3866/PKU.DXHX202212017

Abstract

Abstract:

Herein we describe a comprehensive experiment designed for laboratory teaching of analytical chemistry. The experiment was developed based on the results of one of our recent studies. Methyl viologen (MV²⁺) and 8-hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt (pyranine) were used as the heparin receptor and optical indicator, respectively. Once heparin was introduced in the reaction system, competitive binding led to pyranine displacement, thereby resulting in changes in the UV-Vis absorbance of the pyranine/MV²⁺ system. Using the changes in UV-Vis absorption as the output signal, heparin concentration can be accurately measured using the IDA-based method.

Key words: Analytical chemistry, Comprehensive design experiment, Indicator displacement assay (IDA), Heparin

Zipin Zhang, Zuzhi Zhao. An Indicator Displacement Assay (IDA)-based Analytical Method for Heparin Detection: A Comprehensive Experiment in Analytical Chemistry[J].University Chemistry, 2023, 38(8): 240-247.

Figures/Tables 5

References 26

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An Indicator Displacement Assay (IDA)-based Analytical Method for Heparin Detection: A Comprehensive Experiment in Analytical Chemistry

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