University Chemistry ›› 2018, Vol. 33 ›› Issue (12): 67-70.doi: 10.3866/PKU.DXHX201804029

• Chemistry Laboratory • Previous Articles     Next Articles

Extraction and Determination of Flavonoids from Broussonetia Papyrifera (L.) Vent. Leaves: A Comprehensive Chemical Experiment for College Students

Yuanmei CHEN*(),Laixin DING   

  • Received:2018-04-24 Published:2018-12-14
  • Contact: Yuanmei CHEN
  • Supported by:


A new comprehensive chemical experiment for college students is introduced. This experiment is from the achievements of scientific research. It includes obtaining absorption spectrum and calibration curve of Rutin, and ultrasonic-assisted ethanol extraction and spectrophotometric determination of flavonoids from Broussonetia papyrifera (L.) Vent. Leaves. The experiment involves several basic analytical chemistry procedures and the effective components in plant sample are analyzed. Through the experiment, the students' experimental skills, comprehensive and innovative abilities are improved; furthermore, the specialty requirements are satisfied in agriculture or forestry universities. Thus, it is recommended to be included in the comprehensive analytical chemistry laboratory for students of non-chemistry majors.

Key words: Broussonetia papyrifera (L.) Vent., Flavonoids, Utrasonic-assisted extraction, UV-Vis spectroscopy, Comprehensive chemical experiment