Abstract: This paper presents a comprehensive instrumental analysis experiment featuring online monitoring of 3,5-diamino-1,2,4-triazole (DAT) synthesis via Raman spectroscopy and the exploration of the reaction mechanism. Highlighting a synthesis that integrates instrumental analysis with scientific research, the experiment encompasses DAT synthesis, online reaction monitoring, and mechanism analysis through Raman spectroscopy. By employing chemometric techniques for the analysis of Raman spectral data, the study offers profound insights into the synthesis process, deepening the understanding of the reaction mechanism. This experiment reinforces students’ chemical knowledge, improves their practical laboratory skills, ignites their passion for scientific investigation, and develops their capabilities for research and inquiry.

Key words: Raman spectroscopy, Online monitoring, Synthesis reaction mechanism, Comprehensive experiment