Abstract: In recent years, gold nanoparticles (AuNPs) have been identified as possessing nanozyme properties, enabling them to mimic the catalytic activity of natural peroxidases in the decomposition of hydrogen peroxide (H₂O₂). In this study, AuNPs were synthesized using gelatin as a reducing agent, and the Michaelis constant for the AuNP-catalyzed decomposition of hydrogen peroxide was determined using UV-Vis spectroscopy. Integrating the innovative concept of “nanozyme” into a physical chemistry kinetics experiment designed for undergraduate students not only enhances their comprehensive skills but also fosters their interest in chemical experimentation and instills a sense of national pride.

Key words: Nanozyme, Gold nanoparticle, Hydrogen peroxide decomposition, Michaelis constant, Physical chemistry experiment