通过实验教学加深对理论知识的理解——以“气相色谱实验”教学为例

doi:10.3866/PKU.DXHX201906063

Abstract

Abstract:

Instrumental analysis is a required course for the students of chemistry major. The basic concepts and principles involved in this course are abstract and hard to understand. In this paper, we take gas chromatography experiment as an example to help the students to understand the knowledge by studying the effects of important parameters including column temperature, carrier gas flow-rate, injection volume, and split ratio of capillary gas chromatography on the separation resolution. These experiments could help the students to improve their abilities of analyzing and solving problems and scientific research thinking as well.

Key words: Instrumental analysis, Gas chromatography, Separation resolution, Experimental conditions

Junhong Qian,Ping Hu,Lei Zhou,Wenqing Zhang,Dongxuan Zou,Yan Wang. Better Understanding Theoretical Knowledge through Laboratory Teaching: Taking "Gas Chromatography Experiment" as an Example[J].University Chemistry, 2020, 35(3): 61-66.

柱温	t_M/min	t_R/min					R_{2, 3}	对称因子
柱温	t_M/min	峰1	峰2	峰3	峰4	峰5	R_{2, 3}	峰1	峰5
60 ℃	0.561	1.638	2.799	3.639	5.528	7.659	3.056	1.462	0.481
70 ℃	0.553	1.384	2.216	2.752	4.059	5.340	2.120	1.538	0.673
80 ℃	0.554	1.189	1.808	2.155	3.076	3.850	1.556	1.562	1.123

N₂流量/ (mL∙min⁻¹	t_M/min	t_R/min					R_{2, 3}	对称因子
N₂流量/ (mL∙min⁻¹	t_M/min	峰1	峰2	峰3	峰4	峰5	R_{2, 3}	峰1	峰5
16.3	0.553	1.384	2.216	2.752	4.059	5.340	2.120	1.538	0.673
22.0	0.430	1.101	1.743	2.155	3.157	4.125	2.512	1.483	0.787
28.1	0.364	0.881	1.416	1.758	2.593	3.400	2.049	1.439	0.882

分流比	t_M/min	t_R/min					R_{2, 3}	对称因子
分流比	t_M/min	峰1	峰2	峰3	峰4	峰5	R_{2, 3}	峰1	峰5
32	0.991	1.121	1.223	1.275	1.422	1.526	0.833	1.048	1.073
50	1.497	1.612	1.767	1.843	2.058	2.216	1.269	1.050	1.069
68	2.042	2.287	2.507	2.615	2.922	3.148	1.765	1.063	1.053

组分	分流比(32)		分流比(50)		分流比(68)
组分	理论塔板数	有效塔板数	理论塔板数	有效塔板数	理论塔板数	有效塔板数
组分一	5742	77	12272	62	23708	272
组分二	6365	229	14080	329	27320	940
组分三	6547	325	14328	505	28065	1348
组分四	7605	699	13877	1031	26867	2438
组分五	9943	1222	19377	2040	38201	4715

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Better Understanding Theoretical Knowledge through Laboratory Teaching: Taking "Gas Chromatography Experiment" as an Example

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