目标导向型研究生仪器分析实验教学设计——以GC & GC-MS法测定冰片的成分及含量为例

doi:10.3866/PKU.DXHX202110045

Abstract

Abstract:

Postgraduate course teaching is the first stage of postgraduate training. This article introduces an experiment "GC & GC-MS Determination of Borneol Composition and Content" for postgraduates in the "Comprehensive Instrumental Analysis Laboratory" course. According to the background of students and teaching goals of postgraduate experiments, a student-centered, goal-oriented teaching mode is adopted. Students are required to adopt five experimental routes to achieve the goal through collaboration in the experimental process. In the experiment, students can exercise the scientific and logical way of thinking and the ability to solve complex problems, which lays a solid foundation for future scientific research.

Key words: Gas chromatography, GC-MS, Borneol, (+)-Borneol, Chiral separation, Goal-oriented teaching mode, Mixed teaching

Wei Shao, Wanqun Zhang, Wanqun Hu, Weiwei Li, Kaiping Yang, Pingping Zhu. Teaching Design of the Goal-Oriented Graduate Student Instrumental Analysis Experiment "GC & GC-MS Determination of the Composition and Content of Borneol"[J].University Chemistry, 2022, 37(4): 2110045.

Figures/Tables 13

References 12

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序号	课程内容	课时	授课方式
1	X射线衍射分析	5	讲授+实践
2	核磁共振分析	5	讲授+实践
3	红外光谱分析	5	讲授+实践
4	电感耦合等离子体质谱分析	5	讲授+实践
5	气相色谱及气质联用分析	5	讲授+实践
6	液相色谱-三重四级杆质谱	5	讲授+实践
7	扫描电镜	5	讲授+实践
8	差热与热重分析	5	讲授+实践
9	研讨课	5	讨论

序号	实验路线
任务1	选择合适的分离条件，对龙脑，异龙脑进行分离并确定其各自的保留时间
任务2	使用手性色谱柱，选择合适的分离条件，对左旋/右旋龙脑进行分离
任务3	本实验中有一瓶溶液的标签是错的，试着将它找出来
任务4	有一个样品成分不清楚，请你综合利用所学知识，鉴定其中都有哪些组分
任务5	请你综合利用所学知识，建立方法测定某滴眼液中右旋龙脑的含量

仪器名称	型号	色谱柱
气相色谱仪1	Agilent 7890B GC	TR-WAX 30 m × 0.32 mm × 0.25 µm色谱柱
气相色谱仪2	Agilent 7890B GC	CycloSil-B 30 m × 0.32 mm × 0.25 µm手性柱
气质联用仪	Thermo Fisher ISQ GC-MS	TR-1 MS 30 m × 0.25 mm × 0.25 µm色谱柱

序号	浓度/(mg∙mL⁻¹)	峰面积
标样1	0.4999	357.3
标样2	0.9997	897.5
标样3	1.9994	2174
标样4	2.9991	4203
标样5	3.9988	5792
6号样	x	1897.5

时段	工作	用时
课前	了解实验内容	5 min
课前	了解仪器结构与工作原理	15–20 min
课前	学习工作站操作	15–20 min
课中	实验操作	180 min
课后	实验数据处理	20 min
课后	撰写实验报告	30 min
课后	PPT答辩及内容分享	时间另定

Teaching Design of the Goal-Oriented Graduate Student Instrumental Analysis Experiment "GC & GC-MS Determination of the Composition and Content of Borneol"

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