大学化学 >> 2026, Vol. 41 >> Issue (3): 200-207.doi: 10.12461/PKU.DXHX202507116

所属专题： 化学实验标准与规范建设

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分光光度法及分光光度计使用操作规范建议

王秀云1, 赵发琼2, 刘欲文2, 李一峻3, 范勇4, 刘冬成5, 宋娟娟6, 任艳平7, 李婉8, 范永仙9, 曾秀琼10, 章文伟11, 石梅12, 胡敏13, 邱晓航3, 李维红14, 张剑荣11, 张树永15   

  1. 1 大连理工大学化学学院, 化学国家级实验教学示范中心(大连理工大学), 辽宁 大连 116024;
    2 武汉大学化学与分子科学学院, 化学国家级实验教学示范中心(武汉大学), 武汉 430072;
    3 南开大学化学学院, 化学国家级实验教学示范中心(南开大学), 天津 300071;
    4 吉林大学化学学院, 化学国家级实验教学示范中心(吉林大学), 长春 130012;
    5 广西师范大学化学与药学学院, 化学国家级实验教学示范中心(广西师范大学), 广西 桂林 541004;
    6 内蒙古民族大学化学与材料学院, 化学国家级实验教学示范中心(内蒙古民族大学), 内蒙古 通辽 028000;
    7 厦门大学化学化工学院, 化学国家级实验教学示范中心(厦门大学), 福建 厦门 361005;
    8 中国科学技术大学化学与材料科学学院, 化学国家级实验教学示范中心(中国科学技术大学), 合肥 230026;
    9 浙江工业大学化学工程学院, 化学化工国家级实验教学示范中心(浙江工业大学), 杭州 310014;
    10 浙江大学化学系, 化学国家级实验教学示范中心(浙江大学), 杭州 310058;
    11 南京大学化学化工学院, 化学国家级实验教学示范中心(南京大学), 南京 210023;
    12 复旦大学化学系, 化学国家级实验教学示范中心(复旦大学), 上海 200433;
    13 西安交通大学化学学院, 西安 710049;
    14 北京大学化学与分子工程学院, 化学国家级实验教学示范中心(北京大学), 北京 100871;
    15 山东大学化学与化工学院, 济南 250100
  • 收稿日期:2025-07-28 录用日期:2025-08-27 发布日期:2026-03-24
  • 通讯作者: 李一峻, 任艳平, 张剑荣, 张树永 E-mail:E-mails: yijunli@nankai.edu.cn;ypren@xmu.edu.cn;jrzhang@nju.edu.cn;syzhang@sdu.edu.cn Yijun Li, Yanping Ren, Jinarong Zhang, Shuyong Zhang
  • 基金资助:
    教育部虚拟教研室建设试点项目；教育部高校教师教学组织和教学发展体系建设研究项目

Spectrophotometry and Suggestions on Operating Specifications for Spectrophotometer

Xiuyun Wang1, Faqiong Zhao2, Yuwen Liu2, Yijun Li3, Yong Fan4, Dongcheng Liu5, Juanjuan Song6, Yanping Ren7, Wan Li8, Yongxian Fan9, Xiuqiong Zeng10, Wenwei Zhang11, Mei Shi12, Min Hu13, Xiaohang Qiu3, Weihong Li14, Jinarong Zhang11, Shuyong Zhang15   

  1. 1 National Demonstration Center for Experimental Chemistry Education (Dalian University of Technology), School of Chemistry, Dalian University of Technology, Dalian 116024, Liaoning Province, China;
    2 National Demonstration Center for Experimental Chemistry Education (Wuhan University), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
    3 National Demonstration Center for Experimental Chemistry Education (Nankai University), College of Chemistry, Nankai University, Tianjin 300071, China;
    4 National Demonstration Center for Experimental Chemistry Education (Jilin University), College of Chemistry, Jilin University, Changchun 130012, China;
    5 National Demonstration Center for Experimental Chemistry Education (Guangxi Normal University), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China;
    6 National Demonstration Center for Experimental Chemistry Education (Inner Mongolia Minzu University), College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao 028000, Inner Mongolia Autonomous Region, China;
    7 National Demonstration Center for Experimental Chemistry Education (Xiamen University), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China;
    8 National Demonstration Center for Experimental Chemistry Education (University of Science and Technology of China), School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China;
    9 National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Zhejiang University of Technology), College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
    10 National Demonstration Center for Experimental Chemistry Education (Zhejiang University), Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
    11 National Demonstration Center for Experimental Chemistry Education (Nanjing University), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
    12 National Demonstration Center for Experimental Chemistry Education (Fudan University), Department of Chemistry, Fudan University, Shanghai 200433, China;
    13 School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, China;
    14 National Demonstration Center for Experimental Chemistry Education (Peking University), College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
    15 School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
  • Received:2025-07-28 Accepted:2025-08-27 Published:2026-03-24
  • Contact: Yijun Li, Yanping Ren, Jinarong Zhang, Shuyong Zhang E-mail:E-mails: yijunli@nankai.edu.cn;ypren@xmu.edu.cn;jrzhang@nju.edu.cn;syzhang@sdu.edu.cn

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摘要： 分光光度法是基于物质对紫外、可见等光谱区辐射的选择性吸收特性进行定性与定量分析的光谱分析法，具有操作简便、灵敏度高、测定快速及应用广泛等特点。分光光度计是实现该方法的仪器，其规范操作是化学类等专业学生必备的实验技能。本文主要介绍紫外-可见分光光度法(简称分光光度法)的基本原理及分光光度计的使用方法，并提出相应的操作规范建议。

关键词: 化学实验, 分光光度法, 分光光度计, 操作规范

Abstract: Spectrophotometry is a spectroscopic analytical method based on the selective absorption of electromagnetic radiation of ultraviolet, visible, and other electromagnetic radiation by substances, used for both qualitative and quantitative analysis. It is characterized by operational simplicity, high sensitivity, rapid measurement, and wide applicability. The spectrophotometer is the instrument used to perform this method, and its proper operation is an essential experimental skill for students majoring in chemistry and related disciplines. This article mainly introduces the fundamental principles of ultraviolet-visible (UV-Vis) spectrophotometry (commonly referred to simply as spectrophotometry) and the operation of spectrophotometer, and provides suggestions on operating specifications.

Key words: Chemical experiment, Spectrophotometry, Spectrophotometer, Operating standards