Abstract: Pharmacy is a multidisciplinary field that encompasses biology, chemistry, and medicine. Serving as a fundamental bridge connecting biology and chemistry, biochemistry is a core course in pharmaceutical education and plays a crucial role in cultivating high-level innovative pharmaceutical talents. However, it is traditionally taught in independent chapters with extensive and fragmented theoretical content, making it challenging for students to comprehend and apply flexibly. Effectively integrating fundamental biochemistry theory with pharmaceutical practice to cultivate innovative and practically skilled pharmaceutical professionals is a key topic in the ongoing reform of pharmaceutical education. This study uses activity evaluation of demethylase LSD1 inhibitors as an example and adopts a hybrid teaching model that combines traditional theoretical instruction with case-based teaching. By systematically linking biochemical concepts with the drug development process, this approach enhances students’ engagement, fosters scientific research and innovation, and improves their ability to analyze and solve practical problems in drug discovery. This study explores the application of a closed-loop case-based teaching model in integrating biochemistry and pharmaceutical education, aiming to enhance students’ analytical thinking and problem-solving ability. The findings provide valuable insights for the development of high-level innovative pharmaceutical talent.

Key words: Biochemistry, Pharmaceutical talent training, Closed-loop case-based education, Integrated application, Research innovation