C18反相色谱固定相的制备及性能评价——介绍一个仪器分析拓展性实验

doi:10.3866/PKU.DXHX201810011

Abstract

Abstract:

An extended instrumental analysis experiment "preparation and performance evaluation of C₁₈ stationary phase for reversed-phase chromatography" is recommended for undergraduates. The experiment includes the synthesis, characterization, preparation and properties of the stationary phase. The knowledge of organic synthesis, physical chemistry and instrumental analysis is covered in the experiment. With the practice of the experiment, the typical instrumental analysis experiment is extended. It is helpful for students to cultivate the ability of applying knowledge comprehensively and exercise the ability of scientific research.

Key words: Instrumental analysis experiment, Chromatography, Stationary phase preparation, Performance evaluation

Lihong NIU,Yun ZHOU,Ying YU,Yuan ZHANG,Bilun WU,Weiqi MAI. Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment[J].University Chemistry, 2019, 34(5): 46-50.

样品	保留时间t_R/min		半峰宽W_h_/2/min		理论塔板数N/m		拖尾因子T
样品	商品柱	自制柱	商品柱	自制柱	商品柱	自制柱	商品柱	自制柱
苯	3.31	3.44	0.123	0.171	3999	2245	1.872	1.940
甲苯	4.11	4.49	0.151	0.191	4119	3058	0.944	1.986
联苯	6.37	7.51	0.190	0.262	6218	4558	1.003	2.215

1	沈爱金; 郭志谋; 梁鑫淼. 化学进展, 2014, 26 (1), 10.
2	陈培榕; 李景虹; 邓勃. 现代仪器分析实验与技术, 北京: 清华大学出版社, 2005, 203- 205.
3	武汉大学. 分析化学, 北京: 高等教育出版社, 2007, 468- 471.
4	Neue U. D. ; Alden B. A. ; Walter T. H. J. Chromatogram 1999, 849 (1), 101. doi: 10.1016/S0021-9673(99)00434-3
5	张维冰; 高羽; 彭丽; 赵祎曼. 分析化学, 2016, (6), 835.
6	唐意红; 朱道乾; 关亚风. 分析化学, 2001, (10), 1228. doi: 10.3321/j.issn:0253-3820.2001.10.030

[1]	Wanqun Zhang, Wei Shao, Kaiping Yang, Wanqun Hu. In Situ High-Temperature X-ray Diffraction Study of Titanium Dioxide Phase Transformation: An Extended Instrumental Analysis Experiment [J]. University Chemistry, 2023, 38(8): 186-191.
[2]	Tianyao Xie, Shiying Huang, Ailing Deng, Hua Xiao. Development of Virtual Simulation Software "Electronic Book" for Online-Merge-Offline Experimental Teaching: Taking Capillary Electrophoresis Experiment as an Example [J]. University Chemistry, 2023, 38(8): 209-215.
[3]	Tingting Huang, Ruonan Wang, Zhan Gao, Jichao Sun, Ying Ma, Heng Zhang, Chunjiang Jia, Zhenghu Xu, Shiling Yuan, Gang Liu. Improving the Experiment of Extraction and Separation of Pigments from a Spinach Leaf [J]. University Chemistry, 2023, 38(8): 259-267.
[4]	Weihong Li, Lei Lei. Analytical Instruments in Chemical Experiment Textbooks for Undergraduate Chemistry Majors [J]. University Chemistry, 2023, 38(6): 82-86.
[5]	Juan Zhang, Qingpu Liu, Jingwei Lei, Caixia Xie, Yongsheng Ji, Qiujuan Ma. Practical Applications of Blended Teaching Mode in Instrumental Analysis Course: Taking the "Gas Chromatography Method" as an Example [J]. University Chemistry, 2023, 38(5): 13-20.
[6]	Fang Chen, Hong Wang, Min Liu, Nan Wang, Lihua Zhu. Reform and Practice of Analytical Chemistry Experiment Teaching Based on First-Class Curriculum Construction [J]. University Chemistry, 2023, 38(5): 26-30.
[7]	Zhenbo Liu, Jiayue Xiao, Shuang Liu, Wenshuo Jiang, Xinyu Lan, Wenxuan Jia, Tianyu Li. A Comprehensive Experiment of BODIPY Fluorescent Probes Synthesis and Hypochlorous Acid Detection [J]. University Chemistry, 2023, 38(5): 193-201.
[8]	Siyuan Li, Jiahui Li, Taiheng Liu, Zhaobin Chen, Bin Ren. Self-Built Ion Chromatography and Its Application in Testing Inorganic Anions [J]. University Chemistry, 2023, 38(4): 311-318.
[9]	Yaoyao Zhou, Jiang'ai Long, Ying Wang, Xi Wang, Rui Wang, Haojing Wang, Ruitong Ma, Linyu Tan, Siqi Ma, Yan Li, Lingyan Liu. Expansion and Application of the Instrumental Analysis Experimental Course: Determination of Anions in Drinking Water through Ion Chromatography [J]. University Chemistry, 2023, 38(2): 154-162.
[10]	Tianyao Xie, Ailing Deng, Hua Xiao, Fang Zhu. Study on the Influence of the Substitution Degree of Hydroxypropyl-β-Cyclodextrin in Chiral Capillary Electrophoresis Experiments [J]. University Chemistry, 2023, 38(1): 187-191.
[11]	Huimin Guo, Xin Liu, Yongce Zhang, Yan Su, Wenfeng Jiang, Changgong Meng. Combining Theoretical Investigation with Experiments for Cultivation of Top Innovative Talents: Taking Separation of Alcohols with Gas Chromatography as an Example [J]. University Chemistry, 2023, 38(1): 41-47.
[12]	Yi Wu, Rui Liu, Yi Lv. Renaissance of Gemtuzumab Ozogamicin [J]. University Chemistry, 2022, 37(9): 2206066-.
[13]	Jieyu Zhao, Biqing Shi, Yulian Quan, Pei Pei, Yanfen Zheng. Exploration and Research on Student-Centered Instrumental Analysis Teaching: Taking Introduction to Chromatography as an Example [J]. University Chemistry, 2022, 37(7): 2109015-.
[14]	Guosheng Chen, Shaohan Wang, Yuemei Chen, Haozhi Xie, Hua Xiao, Tao Chen, Ludan Yu, Houjin Li, Fang Zhu, Gangfeng Ouyang. Rapid Analysis of Trace BTEX in Water by Solid Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry [J]. University Chemistry, 2022, 37(5): 2111066-.
[15]	Yan Lü. Reform of Modern Instrumental Analysis Experiment Course for Postgraduates under the New Situation [J]. University Chemistry, 2022, 37(4): 2107077-.

Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

RichHTML

PDF

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 6

Related Articles 15

Metrics

Recommended 0

Preparation and Performance Evaluation of C18Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

RichHTML

PDF

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 6

Related Articles 15

Metrics

Recommended 0

Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment