University Chemistry ›› 2019, Vol. 34 ›› Issue (5): 46-50.doi: 10.3866/PKU.DXHX201810011

• Chemistry Laboratory • Previous Articles     Next Articles

Preparation and Performance Evaluation of C18Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

Lihong NIU*(),Yun ZHOU,Ying YU,Yuan ZHANG,Bilun WU,Weiqi MAI   

  • Received:2018-10-15 Accepted:2018-10-31 Published:2018-11-28
  • Contact: Lihong NIU
  • Supported by:


An extended instrumental analysis experiment "preparation and performance evaluation of C18 stationary phase for reversed-phase chromatography" is recommended for undergraduates. The experiment includes the synthesis, characterization, preparation and properties of the stationary phase. The knowledge of organic synthesis, physical chemistry and instrumental analysis is covered in the experiment. With the practice of the experiment, the typical instrumental analysis experiment is extended. It is helpful for students to cultivate the ability of applying knowledge comprehensively and exercise the ability of scientific research.

Key words: Instrumental analysis experiment, Chromatography, Stationary phase preparation, Performance evaluation