C18反相色谱固定相的制备及性能评价——介绍一个仪器分析拓展性实验

doi:10.3866/PKU.DXHX201810011

Abstract

Abstract:

An extended instrumental analysis experiment "preparation and performance evaluation of C₁₈ stationary phase for reversed-phase chromatography" is recommended for undergraduates. The experiment includes the synthesis, characterization, preparation and properties of the stationary phase. The knowledge of organic synthesis, physical chemistry and instrumental analysis is covered in the experiment. With the practice of the experiment, the typical instrumental analysis experiment is extended. It is helpful for students to cultivate the ability of applying knowledge comprehensively and exercise the ability of scientific research.

Key words: Instrumental analysis experiment, Chromatography, Stationary phase preparation, Performance evaluation

Lihong NIU,Yun ZHOU,Ying YU,Yuan ZHANG,Bilun WU,Weiqi MAI. Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment[J].University Chemistry, 2019, 34(5): 46-50.

样品	保留时间t_R/min		半峰宽W_h_/2/min		理论塔板数N/m		拖尾因子T
样品	商品柱	自制柱	商品柱	自制柱	商品柱	自制柱	商品柱	自制柱
苯	3.31	3.44	0.123	0.171	3999	2245	1.872	1.940
甲苯	4.11	4.49	0.151	0.191	4119	3058	0.944	1.986
联苯	6.37	7.51	0.190	0.262	6218	4558	1.003	2.215

1	沈爱金; 郭志谋; 梁鑫淼. 化学进展, 2014, 26 (1), 10.
2	陈培榕; 李景虹; 邓勃. 现代仪器分析实验与技术, 北京: 清华大学出版社, 2005, 203- 205.
3	武汉大学. 分析化学, 北京: 高等教育出版社, 2007, 468- 471.
4	Neue U. D. ; Alden B. A. ; Walter T. H. J. Chromatogram 1999, 849 (1), 101. doi: 10.1016/S0021-9673(99)00434-3
5	张维冰; 高羽; 彭丽; 赵祎曼. 分析化学, 2016, (6), 835.
6	唐意红; 朱道乾; 关亚风. 分析化学, 2001, (10), 1228. doi: 10.3321/j.issn:0253-3820.2001.10.030

[1]	Junyu Peng, Feng Wang, Hongmei Yuan, Xiaoli Sun. Exploration of the “Sheep-Flock Effect” Teaching Model Based on Dual Preview: Taking Instrumental Analysis Experiment Courses in Local Universities as an Example [J]. University Chemistry, 2025, 40(9): 310-317.
[2]	Runjie Li, Hang Liu, Xisheng Wang, Wanqun Zhang, Wanqun Hu, Kaiping Yang, Qiang Zhou, Si Liu, Pingping Zhu, Wei Shao. Innovative Experiments on Amino Acid Derivation and Chiral Gas Chromatographic Separation [J]. University Chemistry, 2025, 40(6): 286-295.
[3]	Houjin Li, Lin Wu, Xingwen Sun, Yuan Zheng, Zhanxiang Liu, Shuanglian Cai, Ying Xiong, Guangao Yu, Qingwen Liu, Jie Han, Xin Du, Chengshan Yuan, Qihan Zhang, Jianrong Zhang, Shuyong Zhang. Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments [J]. University Chemistry, 2025, 40(5): 93-105.
[4]	Lilong Gao, Yuhao Zhai, Dongdong Zhang, Linjun Huang, Kunyan Sui. Exploration of Thiol-Ene Click Polymerization in Polymer Chemistry Experiment Teaching [J]. University Chemistry, 2025, 40(4): 87-93.
[5]	Qiuting Zhang, Fan Wu, Jin Liu, Zian Lin. Chromatographic Stationary Phase and Chiral Separation Using Frame Materials [J]. University Chemistry, 2025, 40(4): 291-298.
[6]	Siming Bian, Sijie Luo, Junjie Ou. Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase [J]. University Chemistry, 2025, 40(3): 381-386.
[7]	Hua Xiao, Tao Chen, Shui Hu, Jie Li, Zunxiang Zeng, Hongyan Chen, Xianfang Xu, Fang Zhu. Design and Practice of High Performance Liquid Chromatography (HPLC) Disassembly and Assembly Experiment [J]. University Chemistry, 2025, 40(2): 28-39.
[8]	Yurong Deng, Jiahui Yang, Jinyi Zhang, Chengbin Zheng. Application of the Teaching Microplasma Atomic Emission Spectrometer in Innovative Experimental Teaching [J]. University Chemistry, 2025, 40(2): 50-58.
[9]	Yurong Deng, Jiahui Yang, Jinyi Zhang, Chengbin Zheng. Integration of “Course Ideological and Political” Education and “Modular” Instrumental Analysis Experiments: A Case Study of Self-Building and Application of Teaching Microplasma Atomic Emission Spectrometer [J]. University Chemistry, 2025, 40(2): 76-81.
[10]	Zhaobin Chen, Huamin Li, Shunü Peng, Pingping Wu, Shunliu Deng, Yiru Wang. Teaching Design and Practice of Modular Self-Built Ion Chromatography [J]. University Chemistry, 2025, 40(2): 82-88.
[11]	Qian Wu, Yuanxia Lv, Zixuan Guo, Zhihao Zhao, Zhimin Zhang, Hongmei Lu. A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses [J]. University Chemistry, 2025, 40(10): 194-202.
[12]	Shunü Peng, Huamin Li, Zhaobin Chen, Yiru Wang. Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations [J]. University Chemistry, 2025, 40(10): 243-249.
[13]	Jingming Li, Bowen Ding, Nan Li, Nurgul. Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching [J]. University Chemistry, 2024, 39(8): 263-269.
[14]	Tianlong Zhang, Jiajun Zhou, Hongsheng Tang, Xiaohui Ning, Yan Li, Hua Li. Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis [J]. University Chemistry, 2024, 39(6): 295-302.
[15]	Zhenjun Mao, Haorui Gu, Haiyan Che, Xufeng Lin. Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method [J]. University Chemistry, 2024, 39(4): 81-86.

Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

RichHTML

PDF

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 6

Related Articles 15

Metrics

Recommended 0

Preparation and Performance Evaluation of C18Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

RichHTML

PDF

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 6

Related Articles 15

Metrics

Recommended 0

Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment