Abstract:

Because of the important influence of the chiral selector substitution degree on the enantioseparation of ofloxacin in chiral capillary electrophoresis experiments, the effects of the substitution degree of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the inclusion complex between ofloxacin and HP-β-CD were investigated according to different substitution degrees. The results showed that the enantioseparation resolution changed significantly with the substitution degree. Moreover, the best separation resolution was found for a DS (degree of substitution) = 7.9. The methods of determining DS were also discussed. Using these methods, the quality of commercial hydroxypropyl-β-cyclodextrin products can be effectively monitored, and the stability and effectiveness in teaching of the chiral capillary electrophoresis experiment can be ensured.

Key words: Chiral capillary electrophoresis experiment, Substitution degree, Chiral drugs analysis, Hydroxypropyl-β-cyclodextrin, Ofloxacin, Instrumental analysis experiment