University Chemistry ›› 2023, Vol. 38 ›› Issue (1): 187-191.doi: 10.3866/PKU.DXHX202202025

• Chemistry Laboratory • Previous Articles     Next Articles

Study on the Influence of the Substitution Degree of Hydroxypropyl-β-Cyclodextrin in Chiral Capillary Electrophoresis Experiments

Tianyao Xie(), Ailing Deng, Hua Xiao, Fang Zhu   

  • Received:2022-02-12 Accepted:2022-03-24 Published:2022-04-08
  • Contact: Tianyao Xie


Because of the important influence of the chiral selector substitution degree on the enantioseparation of ofloxacin in chiral capillary electrophoresis experiments, the effects of the substitution degree of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the inclusion complex between ofloxacin and HP-β-CD were investigated according to different substitution degrees. The results showed that the enantioseparation resolution changed significantly with the substitution degree. Moreover, the best separation resolution was found for a DS (degree of substitution) = 7.9. The methods of determining DS were also discussed. Using these methods, the quality of commercial hydroxypropyl-β-cyclodextrin products can be effectively monitored, and the stability and effectiveness in teaching of the chiral capillary electrophoresis experiment can be ensured.

Key words: Chiral capillary electrophoresis experiment, Substitution degree, Chiral drugs analysis, Hydroxypropyl-β-cyclodextrin, Ofloxacin, Instrumental analysis experiment