基于共价有机框架的SiO2@COFETTA-Td核壳型固定相的制备及色谱性能研究

doi:10.12461/PKU.DXHX202403031

Abstract

Abstract: This experiment aims to design novel stationary phases for efficient separation of various analytes and apply them in teaching experiments. Covalent organic frameworks (COFs) were coated onto silica microspheres to synthesize core-shell structured composites (SiO₂@COF), which were then employed as a mixed-mode stationary phase for high performance liquid chromatography (HPLC). Thanks to the excellent properties of the covalent organic framework, the alkylbenzenes, anilines, phenols, sulfonamides, nucleosides and polycyclic aromatic hydrocarbons (PAHs), were well separated on the SiO₂@COF packed column. In addition, the SiO₂@COF_ETTA-Td stationary phase was successfully applied in the separation of phthalates, antiepileptic drugs, and polycyclic aromatic hydrocarbons (PAHs) in PM_2.5, further demonstrating its potential for the separation and analysis of complex biological samples. Through this experiment, students can master the operation of HPLC, deepen and consolidate their understanding of chromatography, grasp the principles of core-shell stationary phase separation, and improve their scientific thinking and innovation abilities.

Key words: High performance liquid chromatography, Covalent organic frameworks, Core-shell stationary phase, Instrumental analysis experiment

Fan Wu, Wenchang Tian, Jin Liu, Qiuting Zhang, YanHui Zhong, Zian Lin. Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography[J].University Chemistry, 2024, 39(11): 319-326.

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Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography

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