University Chemistry ›› 2023, Vol. 38 ›› Issue (5): 193-201.doi: 10.3866/PKU.DXHX202207060

• Chemistry Laboratory • Previous Articles     Next Articles

A Comprehensive Experiment of BODIPY Fluorescent Probes Synthesis and Hypochlorous Acid Detection

Zhenbo Liu(), Jiayue Xiao, Shuang Liu, Wenshuo Jiang, Xinyu Lan, Wenxuan Jia, Tianyu Li   

  • Received:2022-07-13 Accepted:2022-09-09 Published:2022-09-23
  • Contact: Zhenbo Liu


Herein, we apply a state-of-the-art fluorescence detection technology from scientific research to teaching. By optimizing the molecular structure and changing the properties of fluorescent probes, we overcome the shortcomings of BODIPY (fluoroboron dipyrrole) fluorescent dyes, such as short emission wavelengths and poor hydrophilicity. A novel hypochlorite fluorescent probe with a fast response, high selectivity, and good sensitivity was synthesized through the design and improvement of the structure of the BODIPY fluorescent dye. The obtained structure of the fluorescent probe was characterized, and the fluorescence properties of the probe were measured. Through the study of the relationship between the probe structure and fluorescence properties, students will have a better understanding of the mechanism of fluorescent probe recognition and the basic principles of fluorescence and luminescence. The experimental part includes organic synthesis and purification, instrumental characterization of the products, and evaluation of the obtained results. This experiment is comprehensive and simple to perform. The methodical experimental procedure is beneficial for cultivating scientific thinking and the students' ability to connect theory with practice. Moreover, this experiment can improve their practical skills, and develop the ability to data process and perform literature research in Chinese and other foreign languages.

Key words: Fluorescent probe, Organic experiment, Instrumental analysis experiment, Comprehensiveness