手性气相色谱法测定野菊花中的龙脑对映体——推荐一个仪器分析实验

doi:10.3866/PKU.DXHX202107023

Abstract

Abstract:

An instrumental analysis experiment "Determination of borneol isomer in wild chrysanthemum by chiral capillary gas chromatography" is recommended. Determination and quantitative analysis of (+)-borneol and (-)-borneol in wild chrysanthemum were conducted using chiral capillary gas chromatographic column in this experiment. Experimental contents consisted of extraction of volatile oil of wild chrysanthemum, optimization of heating program, application of internal standard curve method, etc. From the perspective of analytical chemistry, this experiment focuses on the types and separation principle of chiral chromatographic columns, and it is suggested to be set up as an expanding instrumental analysis experiment.

Key words: Chiral chromatography, Wild chrysanthemum, Borneol isomer, Analytical chemistry experiment

Huixiang Li, Shasha Liu, Jie Lei. Determination of Borneol Isomer in Wild Chrysanthemum by Chiral Gas Chromatography: Recommending an Instrumental Analysis Experiment[J].University Chemistry, 2021, 36(9): 2107023.

Figures/Tables 7

References 16

1	袁黎明; 刘虎威. 色谱手性分离技术及应用, 北京: 化学工业出版社, 2019, 1- 11.
2	林国强; 孙兴文; 陈耀全; 李月明; 陈新滋. 手性合成——不对称反应及其应用, 北京: 科学出版社, 2013, 1- 3.
3	方雪明; 章慧; 陈雷奇; 王宪营; 吴振奕; 董振荣. 大学化学, 2006, 21 (2), 48.
4	张道; 牛国兴; 高翔. 大学化学, 2012, 27 (3), 41.
5	谢芳; 张振锋; 章烨; 张万斌. 大学化学, 2019, 34 (4), 55.
6	王晓康; 李昊昆; 邹思远; 孙兴文. 大学化学, 2020, 35 (4), 27.
7	Chen X. Y. ; Sun L. S. ; Gao X. ; Sun X. W. J. Chem. Educ 2015, 92 (4), 714.
8	谢天尧; 刘丹; 赖瑢; 戴宗; 陈六平; 巢晖. 大学化学, 2014, 29 (6), 38.
9	盛思敏; 沈珍; 陈建成. 大学化学, 2020, 35 (11), 90.
10	Gil-Av E. ; Feibush B. ; Charler-Sigler R. Tetrahedron Lett 1966, 7 (10), 1009.
11	Sand D. M. ; Schlenk H. Anal. Chem 1961, 33 (11), 1624.
12	史雪岩; 王敏; 傅若农. 分析测试学报, 2001, 20 (6), 75.
13	尹明明; 史雪岩; 边庆华; 王敏. 分析化学, 2005, 33 (12), 1747.
14	朱炳辉; 梁艺英; 莫金垣; 王立世; 杨晓云. 分析测试学报, 1999, 18 (4), 70.
15	张琼丹; 刘东辉; 林微微; 魏刚; 黄月纯; 冯小权. 中药新药与临床药理, 2012, 23 (3), 338.
16	金恒亮. 手性气相色谱法——环糊精衍生为固定相, 北京: 化学工业出版社, 2007, 1- 30.

龙脑异构体出峰时的温度/℃	异构体名称	保留时间/min	峰面积	峰高/pA	半峰宽/min	分离度
120	(?)-龙脑	10.96	25.57	5.48	0.07246	2.554
	(+)-龙脑	11.28	28.43	5.72	0.07695
130	(?)-龙脑	8.843	24.74	8.18	0.04652	2.102
	(+)-龙脑	9.013	28.17	8.69	0.04892
140	(?)-龙脑	7.723	25.55	11.80	0.03223	1.724
	(+)-龙脑	7.820	28.36	12.50	0.03394
150	(?)-龙脑	7.170	25.74	15.69	0.02450	1.479
	(+)-龙脑	7.233	28.49	16.49	0.02605

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Determination of Borneol Isomer in Wild Chrysanthemum by Chiral Gas Chromatography: Recommending an Instrumental Analysis Experiment

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