Abstract: Ultra-long organic room temperature phosphorescent (UORTP) materials exhibit significant potential in bioimaging, advanced encryption and anti-counterfeiting, and high-sensitivity sensors, owing to their low cost, low toxicity, stimulus responsiveness, and tunable optical properties. In this study, a donor-acceptor (D-A) type molecule, CNBrBCz, was synthesized from 7H-Benzo[c]carbazole (BCz) and 3-bromo-5-fluorobenzonitrile through a typical aromatic substitution reaction. The chemical structure of CNBrBCz was characterized using ¹H nuclear magnetic resonance (NMR), ¹³C NMR, and high-resolution mass spectrometry. Subsequently, BCz and CNBrBCz were separately doped into polymethyl methacrylate (PMMA) films, and the phosphorescence properties of these doped films were investigated. Organic phosphorescence materials represent a crucial area of research in functional materials chemistry. By integrating advanced scientific research methods and property characterization into fundamental chemistry experiments, this study not only enriches the teaching content of chemical experiments but also enhances students’ proficiency in basic experimental operations and the use of precision instruments. Furthermore, it cultivates students’ scientific literacy and innovation capabilities, while allowing them to observe fascinating luminescence phenomena and appreciate the beauty of chemistry.

Key words: Comprehensive chemical experiment, Ultra-long organic room temperature phosphorescence, 7H-Benzo[c]carbazole, Doping, Integration of science and education