Abstract: This paper introduces the design, construction, and application of an open upconversion fluorescence test system. The system consists of a laser light source, a beam turning mirror, a sample chamber, an optical fiber spectrometer, and a computer. The modular design concept allows students to independently construct and debug the instrument, thereby enhancing their understanding of fluorescence quantitative analysis methods, the principles and structure of fluorescence instruments, and stimulating their curiosity. This approach cultivates an innovative spirit and hones practical skills.

Key words: Upconversion fluorescence, Fluorescence test system, Construction and debugging, Experimental design